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? Performed primary analysis of large biological datasets including gene & 
protein annotation for submission to public government database.
? Operated and troubleshot internal Perl Language Annotation and 
Submission data pipelines in a UNIX command line environment. 
? Edit, Transform and Upload Metadata  SQL database via Perl Scripts.
? Functioned as liaison between internal organization data processing team 
and external clients to ensure data QA/QC
? Manually annotate, curate, & review client submitted data in order to ensure 
adherence to organization data format standards 
? Utilize R for data analysis of large datasets and to create visualizations.? Utilize Jira to update and track completion project requirements and 
milestones.
? Create new and update existing technical SOPs on Internal Wiki

Bioinformatics Analyst

? Primary analysis of CosmosID algorithmic output, including exporting, 
formatting, interpretation, and comparison of metagenomic & microbiome 
data.
? Participate in Beta Testing of new CosmosID proprietary metagenomic 
analytical software including creation of in silico training sets.
? Execute Shell and Perl Data Processing Scripts via Command Line in UNIX 
environments.
? Logging into remotely and running applications on both on site & cloud 
based servers e.g 
? Accession and track incoming laboratory samples using LIMS software
? Prepare genomic libraries of bacterial isolate and metagenomic samples for 
whole genome shotgun sequencing
? Identify and implement new methods and products for improvement and 
optimization of DNA Extraction and library construction
? Operate, monitor, and troubleshoot Illumina GAII NGS platform.
? Utilize Agilent Bioanalyzer and ABI ViiA7 RT­PCR to quantify and QC/QA 
genomic library preps.
? Contribute to writing peer reviewed scientific literature.

NGS Specialist/Bioinformatics Analyst

? Developed and balanced complex genetic disease panels for clinical 
applications on Illumina Next Gen Sequencing GA and High Seq. platforms.
? Prepared gene panel libraries for Illumina Sequencing with both PCR 
Pooling and Raindance protocols.
? Functioned as a team leader in the company’s Capillary Sequencing unit.  
Oversaw maintenance, operation, and validation of ABI 3730 DNA 
Analyzers to ensure generation of high quality sequence data.
? Monitored a complex clinical data pipeline at multiple levels. 
? Trained subordinate team members to follow applicable clinical SOPS in 
order to ensure accurate sequencing results.
? Directed team adherence to relevant industrial regulations i.e. CAP&CLIA in
order acquire and maintain licensing status.? Performed primary analysis of ABI generated DNA sequences for the 
purposes of adequate exon coverage and mutation identification.

Technical Specialist

? Utilized tissue culture techniques including propagation and transfection of 
mammalian cell lines.
? Designed and constructed various siRNA knockout, mammalian transfection 
and expression vectors.
? Harvested and purified protein from cells for analysis. 
? Isolated RNA from mammalian tissues and cells for later reverse 
transcription
? Prepared media and samples for daily laboratory operation.
? Coordinated compliance with radioactive and chemical safety regulations.

Laboratory Technician

Find out detailed information about Richard Isom,  in , MD

Richard Isom: in , MD

Richard Isom

JCVI

CosmosID

GeneDX

Pauli Laboratory

Cornell University

UMUC